HaloTagging Protein for Purifcation,Interactions and Imaging

Cited from HaloTag Technology for Protein Purification, Protein Interactions and Imaging

1. The HaloTag protein can be fused with protein of interest. 
2. A family of HaloTag ligands come with different functionalities. 
3. Ligands consist of two parts: a reactive linker and a functional group, such as fluorescent dye or biotin.
4. Binding of the ligand to the HaloTag protein is rapid and irreversible.
5. The HaloTag protein is genetically modified hydrolase that covalently binds hydrolase substrate like the HaloTag ligands.