Tuesday, 6 September 2016

MS2 Tagging

Cited from In Vivo RNA Visualization in Plants Using MS2 Tagging

"This technique involves the tagging of the RNA of interest with repeats of an RNA stem-loop (SL) that is derived from the origin of assembly of the bacteriophage MS2 and recruits the MS2 coat protein (MCP). Thus, expression of MCP fused to a fluorescent marker allows the specific visualization of the SL-carrying RNA."


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