Sunday, 1 May 2016

FUCCI and Nocodazole in Studying Cell Cycle

FUCCI Cell Cycle Sensor

Cdt1 is a DNA replication factor. It licenses for the formation of pre-replication complex.

Geminin is a DNA replication inhibitor.

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Cited from Aphidicolin

Aphidicolin is an antiviral and antimitotic antibiotic extracted from a fungus, and it is produced as the secondary metabolite. It reversibly inhibits DNA polymerase A,D in eukaryotic cells and therefore inhibits eukaryotic nuclear DNA replication. It arrests cells in early S phase. 

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Cited from G1 versus G2 cell cycle arrest after adriamycin-induced damage in mouse Swiss3T3 cells

Adriamycin is a DNA damaging agent, inducing DNA intercalating. Adriamycin is known to arrest cells in G1 or G2 phase.

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Cited from Nocodazole

Nocodazole interferes with polymerization of microtubules, and cells treated with nocodazole arrests in G2- or M- phase. Prolonged nocodazole leads to apoptosis.





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