RNAi siRNA and shRNA

Cited from RNAi (RNA interference) defined

1. Synthetic (siRNA) or single stranded RNA (ssRNA) containing two complementary sequences separated by a non-complementary sequence, which folds back on itself to form a synthetic short hairpin RNA (shRNA).
2. Expressed from a DNA construct which encodes an shRNA molecule. This is the dd (DNA-directed) RNAi approach.

siNT: non-target siRNA

Cited from the paper "A Novel Multiplex Cell Viability Assay for High-Throughput RNAi Screening"


"Nucleus or DNA stain using fluorescent molecules, such as Hoechst 33342, Hoechst 33258, DAPI or other dyes have been long-serving and commonly applied indicators of cellular viability."