$ fastq_quality_filter -h
usage: fastq_quality_filter [-h] [-v] [-q N] [-p N] [-z] [-i INFILE] [-o OUTFILE]
version 0.0.6
[-h] = This helpful help screen.
[-q N] = Minimum quality score to keep.
[-p N] = Minimum percent of bases that must have [-q] quality.
[-z] = Compress output with GZIP.
[-i INFILE] = FASTA/Q input file. default is STDIN.
[-o OUTFILE] = FASTA/Q output file. default is STDOUT.
[-v] = Verbose - report number of sequences.
If [-o] is specified, report will be printed to STDOUT.
If [-o] is not specified (and output goes to STDOUT),
report will be printed to STDERR.
Cited from Fastx toolkit
FASTX-Toolkit only can output files in compressed format, not use them as input.
According to the documentation:
Some tools can compress the output with GZIP (-z).
Only in one reported case can the input be gzipped:
fasta_clipping_histogram.pl
help page includes this:
INPUT_FILE.FA = input file (in FASTA format, can be GZIPped)
Cited from https://www.biostars.org/p/83237/
No comments:
Post a Comment