Friday 31 March 2017

Text On Top of Stacked Barplots

draw the sum value above the stacked bar in ggplot2

Shortcut for Opening Spotlight

Must-Know Spotlight Keyboard Shortcuts for Mac
  • Open Spotlight menu – Command+Space
  • Open Spotlight in the Finder – Command+Option+Space

Thursday 9 March 2017

Protein Domains Recognize Histone Post-transnational Modifications

Cited from the paper "Chromatin structure and the inheritance of epigenetic information"

The discovery of protein domains — including chromodomains, bromodomains, plant homeodomains (PHDs), tudor domains and malignant brain tumour (MBT) domains specifically recognize a defined histone modification.

Wednesday 8 March 2017

Histone Variant H3.3

Cited from the Paper Distinct factors control histone variant H3.3 localization at specific genomic regions

Mammalian cells express three major types of non-centromeric histone H3 variants, H3.1, H3.2, and H3.3. Although histone H3.3 differs from H3.2 and H3.1 at only 4 or 5 amino acids, H3.3 is specifically enriched at transcriptionally active genes and regulatory elements in non-pluripotent cells.

Wednesday 1 March 2017

Method for Producing Genome Signal Track

Cited from the paper "Cooperative Binding of Transcription Factors Orchestrates Reprogramming"

Genome signal tracks of features (TFs, histone marks, ATAC-seq and RNA-seq) were calculated by partitioning the genome into non-overlapping bins of fixed size (100b for TFs, ATAC-seq and RNA-seq, and 25bp for the histone marks). RPKM values were calculated for each bin using the number of sequencing reads that overlap with the corresponding bin. For histone marks, each read was extended by 200 bp in the direction of the alignment.