Cell Line: G1E ER4

Cited from the Paper "Tissue-Specific Mitotic Bookmarking
by Hematopoietic Transcription Factor GATA1"

"To monitor GATA1 localization on a global scale in living, unsynchronized erythroid cells, GATA1-YFP fusion constructs were stably introduced into G1E cells."

"G1E cells are erythroid precursors that lack GATA1 and consequently fail to mature (Weiss et al., 1997). Introduction of
a conditional form of GATA1 (GATA1 fused to the ligand binding
domain of the estrogen receptor [ER]) conveys estradiol (E2)-
dependent erythroid maturation in a manner faithfully reproducing  that of normal erythroid cells."

"GATA1-ER target gene occupancy  and expression closely match that of endogenous GATA1 in  primary erythroblasts, providing a physiological assay for GATA1 function. Both N-terminal and C-terminal YFP fusions of GATA1-ER were
generated to account for potential effects of YFP on GATA1-ER
function. YFP-GATA1-ER and GATA1-ER-YFP were expressed
at levels similar to endogenous GATA1 and were  equally capable of inducing erythroid differentiation when compared to wild-type GATA1."