Cited from the paper "Assays of nucleosome assembly and the inhibition of histone acetyltransferase activity. (11) Digestion of chromatin; and (12) Purification and characterization of DNA after digestion of chromatin"
"Digestion of chromatin by micrococcal nuclease (MNase) provides a relatively simple method for obtaining information about the locations of nucleosomes along DNAstrands. When nuclei in permeabilized cells are exposed to MNase in the presence of a divalent cation, the enzyme makes double-stranded cuts between nucleosomes. Treatment of chromatin substrates with very high concentrations of MNase yields mononucleosome-length DNA prodominantly, while lower concentrations of the enzyme generate one double-stranded cut at intervals of 10 to 50 nucleosomes, depending on the concentration of the enzyme and the substrate. MNase can also make single-stranded DNA cuts at the sites of histone octamers, and, thus, attempts to map the positions of nucleosomes are usually performed with native double-stranded DNA."
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