Cited from How to Use FlowJo’s Script Editor with Index Sorted Data
Index sorting is a method that deposits individual
cells from a heterogenous mixture into wells of a plate (96, 384, and
so on). Sorted cells are usually selected by some criteria (such as GFP+
and CD11b+, but CD3-) and then channeled into an empty well. Cells that
do not meet the specified criteria are shunted to a waste tube.
The cytometer that sorts the cells
will export a file that specifies the locations of sorted cells into
their respective wells by using some sort of keyword (such as
Index_Sort_Positions, Tray_X, Tray_Y, and so on).
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